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raav2 proviral plasmid  (ATCC)


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    Structured Review

    ATCC raav2 proviral plasmid
    Raav2 Proviral Plasmid, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raav2+proviral+plasmid/Plasmid/us08618352-409-30-34
    Average 99 stars, based on 20065 article reviews
    raav2 proviral plasmid - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Method of identifying compounds using a transgenic pig model of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Article Title: Transgenic porcine models of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Plasmid Preparation:

    Article Title: Method of identifying compounds using a transgenic pig model of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Article Title: Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer
    Article Snippet: .. The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). ..

    Article Title: Transgenic porcine models of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Article Title: Production of CFTR -null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer
    Article Snippet: .. The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking Sbf I sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). ..

    Amplification:

    Article Title: Method of identifying compounds using a transgenic pig model of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Article Title: Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer
    Article Snippet: .. The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). ..

    Article Title: Transgenic porcine models of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Article Title: Production of CFTR -null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer
    Article Snippet: .. The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking Sbf I sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). ..

    Polymerase Chain Reaction:

    Article Title: Method of identifying compounds using a transgenic pig model of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Article Title: Production of CFTR-null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus–mediated gene targeting and somatic cell nuclear transfer
    Article Snippet: .. The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). ..

    Article Title: Transgenic porcine models of cystic fibrosis
    Article Snippet: .. FAM: 6-carboxyfluorescein; NFQ: Non Fluorescent Quencher AAV Production The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking SbfI sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). .. Because of AAV genome size constraints, the total length of the targeting vectors is ̃4.5 kb with the NeoR cassettes centrally located (G16-Neo: 5′ targeting arm=1510 bp; 3′ targeting arm=1274 bp; NeoR cassette=1706 bp. dF-Neo: 5′ targeting arm=1475 bp; 3′ targeting arm=1296 bp; NeoR cassette=1706 bp). pAV2-G16-Neo was grown in SURE2 cells (Stratagene) and purified via a CsCl2 method (Sambrook, Fritz, and Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory Press, Plainview, N.Y., 1989). rAAV1 (with AAV2 ITRs) was prepared as previously described (Yan et al., J. Virol.

    Article Title: Production of CFTR -null and CFTR-ΔF508 heterozygous pigs by adeno-associated virus-mediated gene targeting and somatic cell nuclear transfer
    Article Snippet: .. The targeting vector sequences were amplified from pG16-Neo and pdF-Neo by PCR to include flanking Sbf I sites and were subcloned into the rAAV2 proviral plasmid, pAV2 (ATCC 37216). ..



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    ATCC raav2 proviral plasmid
    Raav2 Proviral Plasmid, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raav2+proviral+plasmid/Plasmid/us08618352-409-30-34
    Average 99 stars, based on 1 article reviews
    raav2 proviral plasmid - by Bioz Stars, 2026-10
    99/100 stars
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